Platelets were also subjected to flow cytometry to determine α-granule ... and immunofluorescence staining. Platelets isolated from mice consuming a HFD were more likely to be activated and ...

Cells were then washed with FACS Buffer [1x PBS, 0.2% bovine serum antigen, 1mM EDTA] before incubating with Fc block [99.5 % FACS Buffer, 0.5% normal rat serum, 1 μg/ml 2.4G2 IgG antibody] prior ...

Flow cytometry assessment was used to detect apoptosis ... Cardiac function was assessed by echocardiography. H&E and Masson’s staining were used to evaluate cardiac tissue structure and fibrosis, and ...

To support physicians in treating cancer patients, oncology laboratories can benefit from adopting end-to-end automation of flow cytometry ... reagents, and antibodies to ease inventory management ...

Her diagnosis was confirmed by bone marrow aspirate analysis, chemical staining, flow cytometry, and biopsy. Atypical mast cells were seen, which were positive for CD117, CD9, CD81, CD33, CD13, CD4, ...

Introduction: Antibody maturation in the lymphoid follicle produces ... the indicated concentrations of Tregitopes and analyzed at day six post-stimulation by flow cytometry. The effect of Tregitope ...

Multiplexed immunofluorescence staining, flow cytometry and functional in-vitro experiments were performed for validation. Results SHCC presented with less genomic heterogeneity compared with usual ...

In some experiments, mice received neutralising antibody against HMGB1 (2 mg/kg ... mouse HS model was used and ILC2 expansion in the lungs was dynamically measured by flow cytometry. The percentages ...

Correspondence to Dr Daniel E Stange, Department of Visceral, Thoracic and Vascular Surgery, Medical Faculty, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden 01307, ...