And because the method depends on antibody binding to surface receptors, there’s always the possibility that sorted cell populations will be activated or altered by the process itself. “For the most ...

However, choosing optimal antibody-fluorochrome combinations is a complex process. Here we provide some simple guidelines for the selection of reagent panels for multicolor flow cytometry.

Bio-Rad’s range of StarBright Dyes, proprietary fluorescent nanoparticles conjugated to flow-validated antibodies against ... reproducible and consistent staining and the dyes are resistant ...

We highly encourage you to contact the flow core for help with multi-color panel design before purchasing antibodies and for advice on sorting preparation. All cytometry services are performed at an ...

“If you compare multiple antibodies to different epitopes of a given target, you can very rapidly conclude what’s real staining and what’s not,” says Burmer. Research your reagent ...

Guidelines for working in the Flow Cytometry Unit, user access policy. Also basic flow cytometry and find some help for planning your experiments. User ac­cess policy The Flow Cytometry Unit provides ...

Developed initially in the field of immunology, flow cytometry now has applications throughout the biological sciences and beyond. A successful flow cytometry experiment begins with good sample ...